Methods for determination of serum albumin in the assay of glycated albumin
- VernacularTitle:血清白蛋白检测方法对糖化白蛋白的影响
- Author:
Debao SHI
;
Liying LV
- Publication Type:Journal Article
- Keywords:
glycated albumin;
bromcresol green;
modified bromcresol purple;
immunoturbidmetic assay
- From:
Acta Universitatis Medicinalis Anhui
2015;50(12):1805-1808
- CountryChina
- Language:Chinese
-
Abstract:
A total of 306 fresh human serum samples were randomly selected. Alb concentrations were measured by bromcresol green ( BCG) method, modified bromcresol purple ( mBCP) method, and immunoturbidmetic assay ( ITA) , respectively. GA was measured by an enzymatic method. GA value was expressed as the percentage of GA in the total serum Alb [ GA%(%) =GA/Alb?100%] . When Alb≥40 g/L, the clinical differences between BCG and mBCP, BCG and ITA, and mBCP and ITA were not significant, and there was no statistical difference between GA% BCG and GA% mBCP(P=0. 537); when Alb<40 g/L, BCG had statistical difference between mBCP and ITA ( P<0. 01 ) , and GA% BCG was significantly lower than GA% mBCP ( P<0. 01 ) . No obvious clinical signifi-cance of Alb concentrations was observed measured by BCG, mBCP, and ITA when Alb≥40 g/L. There was no difference in different Alb methods for GA% calculation. When Alb<40 g/L, the consistency of mBCP and ITA was better than that of BCG and ITA, which indicated that mBCP method may be more suitable for the assay of gly-cated albumin value ( GA%) , whereas the GA% may be underestimated if using BCG method for the determination of Alb.
