Effects of Galangin on Gene Expressions of Nrf2 and γ-GCS in Oxidative Damage of A375 Cell Induced by H2O2
10.3969/j.issn.1005-5304.2015.11.019
- VernacularTitle:高良姜素对过氧化氢诱导的A375细胞氧化损伤后Nrf2、γ-GCS基因表达的影响
- Author:
Shixia HUO
;
Xiaoming PENG
;
Li GAO
;
Yi HUANG
;
Ping GAN
;
Ming YAN
- Publication Type:Journal Article
- Keywords:
galangin;
A375;
oxidative damage;
ROS;
Nrf2;
γ-GCS
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;22(11):69-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of galangin on gene expressions of Nrf2 andγ-GCS in oxidative damage of A375 cell;To discuss its protective mechanism for anti-oxidative damage. Methods A375 melanoma cells were induced oxidative stress to establish oxidative damage model by 700μmol/L H2O2. The study was divided into normal group, model group, positive medicine group and high-, medium-, and low-dose galangin groups. All administration groups were given relevant medicine for cultivation. Cell viability was detected by MTT;ROS content was detected by ELISA;the gene expressions of Nrf2 andγ-GCS were detected by RT-PCR.Results Compared with normal group, cell viability decreased significantly;ROS content increased significantly;the gene expressions of Nrf2 andγ-GCS decreased significantly in the model group (P<0.05,P<0.01). Compared with model group, cell viability increased, ROS content decreased, the gene expressions of Nrf2 andγ-GCS increased significantly in all administration groups (P<0.05,P<0.01). Conclusion Galangin may activate Nrf2 signal path to realize the protective effect on A375 cellular oxidation damage through upregulating the expressions of Nrf2 andγ-GCS to promote the integration of Nrf2 and antioxidant response element and relevant regulatory enzymes.
