Expressions and clinical significance of tetraspanin CO-029 in intrahepatic cholangiocarcinoma
10.3760/cma.j.issn.1007-8118.2015.08.005
- VernacularTitle:四跨膜蛋白CO-029在肝内胆管细胞癌中的表达及临床意义
- Author:
Ming ZHAO
;
Zhuori LI
;
Kailun ZHOU
;
Liang CHEN
;
Yehong WANG
;
Jincai WU
- Publication Type:Journal Article
- Keywords:
Intrahepatic cholangiocarcinoma;
Tetraspanin,CO-029;
Metastasis and recurrence;
Prognosis
- From:
Chinese Journal of Hepatobiliary Surgery
2015;21(8):523-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of tetraspanin CO-029 in intrahepatic cholangiocarcinoma (ICC) and to find out their clinical significance.Methods RT-PCR and western blot were used to detect the expressions of CO-029 in ICC and their matched para-tumorous tissues from 20 patients with ICC,as well as in the HCCC-9810 cell lines.The expressions of CO-029 were further detected via tissue microarray (TMA) in the pathological specimens of 40 patients with ICC.Correlations between the expressions of CO-029 and the clinicopathologic features and prognosis were analyzed.Results A high level of CO-029 was detected in the 20 patients with ICC and the HCCC-9810 cell lines via western blot and RTPCR.Moreover,the expression levels of CO-029 in the ICC tissues were higher than the matched para-tumorous tissues (P ＜ 0.05).TMA detection revealed the positive expression rate of CO-029 to be 65％ (26/40).The expression level of CO-029 was much higher in the early recurrence group (Time to recurrence,TTR ＜ 1 year) than the non-recurrence group (TTR≥ 1 year).On analysis,the correlations were significant between the expressions of CO-029 and tumor encapsulation,hilar lymph node metastasis,TNM stage and prognosis (P ＜0.05).Conclusions CO-029 was highly expressed in ICC.It had close correlations with recurrence,metastasis and prognosis of patients with ICC.
