The effects of Apelin-13 on the behavioral and the expression of YAP in the cerebral ischemia/reperfusion injury of rats
10.3760/cma.j.issn.1674-6554.2015.10.005
- VernacularTitle:Apelin-13对脑缺血再灌注大鼠行为学及脑内YAP蛋白表达的影响
- Author:
Xiaoge YAN
;
Liangcai DING
;
Chunmei WANG
;
Baohua CHENG
;
Jing CHEN
;
Bo BAI
- Publication Type:Journal Article
- Keywords:
Apelin-13;
Neuroprotection;
Cerebral ischemia/reperfusion injury;
Behavioral;
Yes-associated protein
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2015;24(10):882-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effect of lateral intracerebroventricular injection of Apelin-13 on the YAP expression and the cerebral ischemia/reperfusion (I/R) injury in Wistar rats.Methods Healthy adult male Wistar rats were randomly divided into the sham group, cerebral I/R group and Apelin-13 treatment group.The middle cerebral artery occlusion model was established with ischemia for 1 hour and reperfusion for 24 hours after restricting food and water intake for 12 hours.Apelin-13 was injected into rats' lateral ventricles of Apelin-13 treatment group after reperfusion.Neural function defects was assessed.The volume of infarction was evaluated by TTC staining.The expression levels of YAP were detected by western blot.Results Compared with the cerebral I/R group,the rats in the Apelin-13 treatment group had abetter neurologic score ((2.67±0.33) vs (1.67±0.33) , P＜0.05), the infarction volume was decreased ((30.60± 1.42) ％ vs (23.70± 2.20) ％,P＜0.05) , and YAP expression level was increased in each part of the cerebral tissue(P＜0.05).Conclusion Apelin-13 has a neuroprotective effect,which plays the therapeutic effect by regulating the expression of YAP on cerebral ischemia/ reperfusion (I/R) injury in Wistar rats.
