Preparation of a deactivation vaccine against Aeromonas hydrophila and research about its immue effect in the north of Henan

VernacularTitle:豫北地区嗜水气单胞菌灭活疫苗的制备及免疫效果的研究
Author: Jianyi GUAN ; Huili MAO ; Wenxu HE ; Limin YANG ; Yundong FU ; Jingmei ZHANG ; Jiajing WU ; Qianji NING
Publication Type:Journal Article
Keywords: Aeromonas hydrophia; Vaccine; Antibody titers; Tissue slices; Immunization protection rate
From: Chinese Journal of Immunology 2015;(11):1501-1504
CountryChina
Language:Chinese
Abstract: Objective:Selected virulence factors more than and high virulent Aeromonas hydrophia strain made into inactivated vaccine,to study the immunization effect of inactivated vaccine.Methods:Crucian were vaccinated with formalin-killed vaccine via in-traperitoneal injection.Controls were injected with the same volumes of saline.Then the antibody titres, histopathology and relative percent survival were analyzed from samples of both groups.Results: The antibody in the indirect agglutination reaction could be detected in vaccinated fish once a weeks after immunization and reached highest level 6 weeks after immunization.The histopathology analysis indicated that the vaccine had a good protective effect on crucian target organs.Vaccinated fish showed 100%relative percent survival and the immune period would be 6 month.Conclusion:The vaccine in this study has a significant protective effect on crucian and may be used as effective fish vaccines against bacterial septicemia.