Effect of peroxisome proliferator-activated receptor γagonist rosiglita-zone on intestinal injury in rats undergoing orthotopic autologous liver transplantation by inhibiting inflammatory response
10.3969/j.issn.1000-4718.2015.09.019
- VernacularTitle:PPARγ激动剂罗格列酮通过抑制炎症反应减轻肝移植大鼠围术期肠损伤
- Author:
Nan CHENG
;
Xinjin CHI
;
Xi LI
;
Mian GE
;
Wanling GAO
;
Ziqing HEI
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferators-activated receptor γ;
Orthotopic autologous liver transplantation;
Intesti-nal injury;
Rosiglitazone;
Inflammatory response
- From:
Chinese Journal of Pathophysiology
2015;(9):1637-1641
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of rosiglitazone , a peroxisome proliferators-activated receptor γ(PPARγ) agonist, on the expression of PPARγ, the activation of NF-κB and intestine injury in the rats undergoing ortho-topic autologous liver transplantation ( OALT ) .METHODS: Sprague-Dawley male rats were randomly divided into 4 groups:control group, sham group, OALT group and rosiglitazone (0.3 mg/kg, iv) pretreatment (ROS+OALT) group. The OALT model was established , and the intestinal tissues were collected 8 h after the liver reperfusion .The intestinal tis-sue sections were stained to visualize the damage .The expression of PPARγand NF-κB in the tissues, the concentrations of diamine oxidase (DAO) and fatty acid-binding protein 2 (FABP2) in the serum and the concentration of TNF-αand IL-6 in the tissues were measured .RESULTS:Compared with sham group , the intestinal mucosa of the rats showed obvious pathological injury after liver reperfusion in OALT group and ROS group , the Chiu’s scores of intestinal mucosa was signifi-cantly higher , and the serum concentrations of DAO and FABP 2 increased ( P<0.05 ) .After rosiglitazone pretreatment , the injury of intestinal mucosa of the rats was alleviated , the Chiu’s scores was lower and the serum concentrations of DAO and FABP2 decreased (P<0.05), the PPARγexpression was obviously up-regulated in the intestinal tissues, the nuclear translocation of NF-κB was reduced and the concentrations of IL-6 and TNF-αwere decreased .CONCLUSION: During perioperative period of OALT in rats , the inflammatory responses are obvious .Furthermore, obvious intestinal injury oc-curs .PPARγagonist rosiglitazone obviously up-regulates PPARγexpression and inhibits the inflammation in the intestines , thus protecting against intestinal injury in rats undergoing OALT .
