Effects of lipoxin A4 on the inflammatory reaction induced by uric acid in human umbilical vein endothelial cells
10.3760/cma.j.issn.1000-6699.2015.09.014
- VernacularTitle:脂氧素 A4对尿酸诱导的人脐静脉内皮细胞炎症反应的影响
- Author:
Tingting YI
;
Jingguo ZHOU
;
Guangcheng LUO
;
You ZHOU
;
Yufeng QING
;
Xingliang JIANG
- Publication Type:Journal Article
- Keywords:
Lipoxin A4;
Uric acid;
Human umbilical vein endothelial cells;
p38 mitogen-activated protein kinase;
NF-κB/p65
- From:
Chinese Journal of Endocrinology and Metabolism
2015;(9):800-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of lipoxin A4 ( LXA4 ) on inflammatory related factors induced by uric acid( UA) in human umbilical vein endothelial cells( HUVECs) . Methods HUVECs were treated with 5, 25, and 50 ng/ml LXA4 prior to exposure to 12 mg/dl UA. Tumor necrosis factor-alpha(TNF-α), interleukin ( IL)-1β, and IL-6 were analyzed with ELISA and realtime PCR. The phosphorylation levels of p38 mitogen-activated protein kinases( MAPK) and NF-κB/p65 were observed with Western blot. Results Stimulation of HUVECs with 12 mg/dl UA markedly increased TNF-α, IL-1β, and IL-6 production(P<0. 01). Pretreatment with LXA4 significantly inhibited UA-induced production of TNF-α, IL-1β, and IL-6 in a concentration dependent manner(P<0. 01). The mRNA expressions of TNF-α, IL-1β, and IL-6 in response to UA were also decreased by LXA4(P<0. 05). Western blot analysis showed that the phosphorylation levels of p38 MAPK and NF-κB/p65 were significantly raised by 12 mg/dl UA in HUVECs(P<0. 05), but attenuated significantly in the presence of 50 ng/ml LXA4. Conclusion LXA4 may inhibit the expressions of inflammatory related factors induced by UA via reducing p38 MAPK and NF-κB/p65 phosphorylation and play a role in anti-inflammation.
