Relationship of ultrasound cavitation pressure amplitude and rat blood brain barrier permeability
10.3760/cma.j.issn.1004-4477.2015.08.022
- VernacularTitle:微泡超声空化声压幅度与大鼠血脑屏障通透性关系的研究
- Author:
Hai CUI
;
Yali XU
;
Shengzheng WU
;
Lu LI
;
Shunji GAO
;
Zheng LIU
- Publication Type:Journal Article
- Keywords:
Blood-brain barrier;
Microbubblest;
Cavitation;
Hemicerebrum;
Rat
- From:
Chinese Journal of Ultrasonography
2015;(8):715-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effectiveness of different peak negative pressure ultrasound irradiated microbubble destruction on the control of rat blood brain barrier permeability.Methods Thirty healthy SD rats were divided into 5 groups according to different ultrasound pressure level (62 kPa,86 kPa, 1 81 kPa,324 kPa,708 kPa).After intravenous injection of microbubble,the rats were underwent ultrasonic irradiation for 9 minutes,and the contralateral hemispheres were served as controls.The permeability of blood brain barrier was determined by the quantitative analysis of Evans blue and lanthanum nitrate extravasation.Results Quantitative analysis results revealed a leakage in Evans blue about(4.52 ± 0.1 1 )μg/g and (10.56±0.13)μg/g in group 1 81 kPa and 708 kPa,respectively.A wide endothelial cell gap with lanthanum nitrate trace leaked out was shown at the group of ultrasound pressure of 1 81 kPa,and erythrocytes extravasations were found when ultrasound pressure at 708 kPa.Conclusions The pressure amplitude in microbubble enhanced cavitation can effectively regulate and control blood-brain barrier opening in rats.
