Application of tissue spontaneous fluorescence in draw focal cerebral ischemia reperfusion injury of rats
10.3969/j.issn.1001-1978.2015.10.029
- VernacularTitle:组织自发荧光在大鼠局灶性脑缺血/再灌注损伤中的应用
- Author:
Shuhong TIAN
;
Richao WANG
;
Guilan XING
;
Jian FU
- Publication Type:Journal Article
- Keywords:
rat;
brain tissue;
autofluorescence;
focal cerebral is-chemia reperfusion;
in vivo imaging system
- From:
Chinese Pharmacological Bulletin
2015;(10):1473-1476
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss the application of tissue spontane-ous fluorescence for draw focal cerebral ischemia reperfusion in-jury in rats based on specific fluorescence detection technology. Methods The change of spontaneous fluorescence WAS COM-PARED between the brains of normal rats and those of rats with draw focal cerebral ischemia-reperfusion injury and an quantita-tive analysis was then made. Result The results showed that spontaneous fluorescence of brain tissue for focal cerebral ische-mia reperfusion injury changed significantly. Spontaneous fluo-rescence signal of injury considerably enhanced. The fluores-cence signal which was quantified by IVIS had significant statisti-cal significance compared with normal brain tissue, P <0. 05. Conclusion Our research shows that spontaneous fluorescence of brain tissue enhances obviously after focal cerebral ischemia-reperfusion injury. Our research provides a method for the re-search and evaluation of focal cerebral ischemia-reperfusion inju-ry model in rats.
