RP-HPLC Determination of Costunolide and Dehydrocostus Lactone in Tibetan Medicine Liuwei Muxiang Pill
10.3969/j.issn.1005-5304.2015.10.026
- VernacularTitle:RP-HPLC测定藏药六味木香丸中木香烃内酯和去氢木香内酯含量
- Author:
Zhenghua JIAO
;
Xiaoshuan LIU
;
Jincao YANG
- Publication Type:Journal Article
- Keywords:
Tibetan medicineLiuwei Muxiang Pill;
Costunolide;
Dehydrocostus Lactone;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(10):94-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method to analyze the contents of Costunolide and Dehydrocostus Lactone in Tibetan medicineLiuwei Muxiang Pill.Methods HPLC method was used; the XDB-C18 column (4.6 mm × 50 mm, 1.8μm) was adopted;the mobile phase was methanol- water (60∶40);the flow rate was 0.8 mL/min;UV detection was 225 nm.Results The linear range of Costunolide and Dehydrocostus Lactone was 0.235 5-1.413μg and 0.291 3-1.748μg. The average recoveries were 99.25% and 99.41%, respectively.Conclusion The method is accurate, reliable, stable and with good repeatability, which provides the basis for the quality evaluation of Tibetan medicineLiuwei Muxiang Pill.
