The cytotoxicity of injectable PHEMA/CPC composite hydrogel and it's effect on cell apoptosis
10.3969/j.issn.1001-3733.2015.06.005
- VernacularTitle:可注射型 PHEMA/CPC 复合水凝胶的细胞毒性及其对细胞凋亡的影响
- Author:
Genjian ZHENG
;
Lan ZHOU
;
Fengfeng WANG
- Publication Type:Journal Article
- Keywords:
PHEMA/CPC;
Hydrogels;
Cytotoxicity;
Cell apoptosis
- From:
Journal of Practical Stomatology
2015;(6):761-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the cytotoxicity of PHEMA /CPC composite hydrogel.Methods:L929 cells were cultured by RP-MI1 640 with 1 0% fetal bovine serum(blank control),with PHEMA /CPC extraction(experimental group),high density polyethy-lene(HDPE)extracts(negative control)and 5% DMSO(positive control)for 24,48 and 72 h respectively.The cell proliferation was examined by MTT assay.Cell apoptosis was detected by flow cytometry and Annexin V-FITC /PI kit.Results:The A value of positive control group decreased with the increase of culture time(P <0.01 )(between experimental and negative control groups,P>0.05).The cytotoxicity of the experimental group was grade Ⅰ.That of other groups increased with the increase of culture time. Cell apoptosis(%)in PHEMA /CPC composite hydrogel group and blank group was 4.21 ±0.30 and 4.89 ±0.39 respectively(P>0.05).Conclusion:Injectable PHEMA /CPC composite hydrogel has no cytotoxicity and no effect on cell apoptosis.
