Ribosomal protein S7 affects apoptosis of HeLa cervical cancer cells
10.3760/cma.j.issn.1673-422X.2015.08.007
- VernacularTitle:核糖体蛋白 S7对宫颈癌 HeLa 细胞凋亡的影响
- Author:
Hui DING
;
Jingxian LING
;
Junhao CHEN
;
Kui ZHANG
;
Qingfei WANG
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Apoptosis;
Ribosomal protein S7
- From:
Journal of International Oncology
2015;(8):585-588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily investigate the effect of ribosomal protein S7 on apoptosis of HeLa cervical cancer cells.Methods The previously constructed recombinant plasmid pIRES2-EGFP-RPS7 was transfected into HeLa cells,the empty vector pIRES2-EGFP transfected cells as control.Enhanced green fluorescent protein(EGFP)expressing cells were quantified by flow cytometry,and RPS7 protein level was also determined by Western blotting.Cell apoptosis of both RPS7 over-expression cells and knockdown cells were evaluated by flow cytometry after staining using allophycocyanin labeled Annexin-V.Results Apoptotic cell level in the obtained RPS7 transient over-expression HeLa cells was significantly higher than that of vector con-trol cells [(1 0.00 ±0.60)% vs (5.73 ±0.61 )%],with a statistic difference (t =8.63,P =0.001 ). Moreover,the apoptotic level in RPS7 knockdown cells was lower than that in control cells [(3.08 ± 0.49)% vs (5.97 ±0.63)%],with a statistic difference (t =6.40,P =0.003).Conclusion Up-regula-tion of RPS7 may promote apoptosis,while down-regulation of RPS7 may inhibit apoptosis of HeLa cells,indi-cating that RPS7 may play roles in regulating cell apoptosis.
