Synthesis and identification of artificial antigens of lung elastin degradation peptide
10.3969/j.issn.1671-8348.2015.22.004
- VernacularTitle:肺弹性蛋白降解肽段人工抗原的合成与鉴定?
- Author:
Fangyi PENG
;
Yuhua CUI
;
Mingyan MA
;
Yuankuan LI
;
Hongyu WU
;
Hairong JIANG
;
Huan ZHOU
- Publication Type:Journal Article
- Keywords:
lung elastin degradation peptide;
artificial antigens;
antibody
- From:
Chongqing Medicine
2015;(22):3037-3039
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize and identify artificial antigens of lung elastin degradation peptide and for the purpose of preparation of COPD test.Methods The artificial antigens were synthesized by Sulfo-SMCC and KLH.The complete antigens were identified by ultraviolet spectrum and SDS-PAGE.Immunize Balb/c mice was used to prepare antibody.The antiserum activity was evaluated by indirect competitive ELISA.Results The artificial antigens were identified by ultraviolet spectrum and SDS-PAGE. The protein concentration was 1.181 mg/mL.The titer of antiserum was 1∶64 000,and IC50 was 13.7 ng/mL.The antiserum had no cross-reaction with nonsense peptide.Conclusion The artificial antigens were acquired successfully,which had good immunoge-nicity.The results have laid basis for COPD test.
