Establishment and Application of TaqMan Real-Time PCR assay for Diagnosis of Streptococcus Pneumoniae
10.3969/j.issn.1671-7414.2014.05.013
- VernacularTitle:肺炎链球菌TaqMan实时荧光定量PCR的建立及临床应用
- Author:
Dongyue LU
;
Helu LIU
;
Yue HE
;
Peipei LI
;
Hong LIANG
;
Shue ZHU
- Publication Type:Journal Article
- Keywords:
TaqMan real-time PCR;
children CAP;
streptococcus pneumonia;
rapid diagnosis
- From:
Journal of Modern Laboratory Medicine
2014;(5):60-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop TaqMan real-time PCR assay for detection and identification of streptococcus pneumonia iso-lated from children CAP.Methods Based on the sequences of lytA gene,primers and probe were designed and the assay was optimized.Then 1 504 sputum samples were detected by culture and the developed assay with double-blind testing.Results The lower limit of detection in the developed real-time PCR assay was 18.75 cfu/PCR,and had no cross reaction.141 strep-tococcus pneumonia strains were detected from 1 504 samples and 140 were isolated by culture.The whole process just nee-ded 2.5 h.Conclusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the i-dentification of streptococcus pneumonia,but also provide evidence for antibiotic therapy.
