Preparative isolation of Heteroclitin D from Kadsurae Caulis using normal-phase flash chromatography
- Author:
Xiaoxue YU
;
Qianwen WANG
;
Xinjun XU
;
Weijian LV
;
Mingqian ZHAO
;
Zhikun LIANG
- Publication Type:Journal Article
- Keywords:
Kadsurae Caulis;
Heteroclitin D;
Normal-phase flash chromatography
- From:
Journal of Pharmaceutical Analysis
2013;(6):456-459
- CountryChina
- Language:Chinese
-
Abstract:
Heteroclitin D (H.D) was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystallized by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.
