Study of Antitumor Effect of Combination of CIK with DC both Pulsed by Breast Cancer Stem Cell Antigen in Mice Model with Tumor
10.3969/j.issn.0253-9896.2014.06.012
- VernacularTitle:负载干细胞抗原的DC联合CIK细胞对乳腺癌荷瘤鼠肿瘤杀伤研究
- Author:
Chunmiao PANG
;
Yan LYU
;
Wenwen SUN
;
Yuling SI
;
Hua PANG
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
stem cells;
dendritic cells;
killer cells;
gene,bcl-2;
bcl-2-associated X protein;
apop-tosis
- From:
Tianjin Medical Journal
2014;(6):554-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the tumor-inhibitory effect of cytokine-induced killer cells(CIK)co-cul-tured with dendritic cells (DC)pulsed by breast cancer stem cell antigen on the same tumor-bearing mice. Methods Breast cancer stem cells were isolated from the cell line of MCF-7/ADR and extract lyses antigen of the stem cell was saved. DC and CIK derived from peripheral blood mononuclear cells of healthy individuals were co-cultured and pulsed or un-pulsed by the above antigen lyses. This DC+CIK were injected to breast tumorbearing mice (BCSC-AP-DC+CIK group), and were used to compared with the common breast cancer cell antigen (rather than breast cancer stem cell antigen) pulsed DC+CIK group(AP-DC+CIK group), DC+CIK group, CIK CIK group and normal saline group(NS group). The tumor-inhibitory effect were evaluated and compared among all 5 groups through the tumor size, TdT-mediated dUTP nick end labeling test (TUNEL), examining expression level of bcl-2 and bax by immunohistochemistry. Results The tumor size in each group before and after therapy and the tumor size after therapy between each group was of significant difference(P＜0.05). The maximum size is NS group(3.625±0.093)cm3 and BCSC-AP-DC+CIK group is minimum,which is (1.234±0.131)cm3. BC-SC-AP-DC+CIK group is of highest expression of bax and apoptotic index value, lowest bcl-2 expression in all 5 groups. Conclusion The CIK co-cultured with DC pulsed breast cancer stem cell antigen was more effective to induce apoptosis of breast cancer cells than those of CIK cells co-cultured with DC pulsed breast cancer cell antigen,CIK cells co-cultured with DC and CIK cells.
