More than 3 ku proteins in chicken egg extract up-regulate expression of pluripotent genes Oct-3/4 and Nanog
10.3969/j.issn.2095-4344.2014.37.023
- VernacularTitle:鸡卵清提取液中大于3 ku蛋白促细胞升高表达多能基因Oct-3/4和Nanog
- Author:
Guangping RUAN
;
Xiang YAO
;
Jufen LIU
;
Fan SHU
;
Jinxiang WANG
;
Jie HE
;
Jianyong YANG
;
Jing ZHAO
;
Rongqing PANG
;
Xinghua PAN
- Publication Type:Journal Article
- Keywords:
chickens;
genes;
hek293 cells
- From:
Chinese Journal of Tissue Engineering Research
2014;(37):6029-6033
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Reprogramming somatic cells to generate pluripotent stem cells has a wide application in biomedical research. OBJECTIVE:To analyze the effect of different molecular weight proteins in chicken egg-white extract to elevate expression of pluripotent genes Oct-3/4 and Nanog in 293T cells. METHODS:The extracts of chicken egg-white were separated into more than 3 ku and less than 3 ku ingredients to be used for co-culture with 293T cells. There were four groups, 1×105 293T cells per wel , total 500μL. In the control group, 500μL culture medium was added;in the other three groups, 500μL chicken egg-white extract, more than 3 ku and less than 3 ku ingredients were respectively added. Quantitative PCR was used to determine the relative expression levels of pluripotent genes Nanog and Oct-3/4 in 293T cells. RESULTS AND CONCLUSION:By using co-culture method, more than 3 ku ingredients have a role to increase the expression of pluripotent genes Oct-3/4 and Nanog, but less than 3 ku ingredients cannot elevate the expression of pluripotent genes. This indicates that the ingredient of chicken egg-white extract to elevate the expression of pluripotent genes is more than 3 ku proteins.
