Comparison of two protein preparation methods by two-dimensional gel electrophoresis
10.7644/j.issn.1674-9960.2014.08.017
- VernacularTitle:双向电泳法比较两种蛋白质组样品制备方法
- Author:
Yong CUI
;
Dongshu WANG
;
Erling FENG
;
Xiankai LIU
;
Hengliang WANG
;
Li ZHU
- Publication Type:Journal Article
- Keywords:
proteomics;
electrophoresis,gel,two-dimensional ( 2-DE );
protein extraction;
ultrasonic disruption;
glass-beads grounding
- From:
Military Medical Sciences
2014;(8):638-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the separation effects of protein samples extracted by two different methods with two -dimensional gel electrophoresis (2-DE) .Methods Ultrasonic disruption and glass-beads grounding were used to prepare protein samples of Gram-negative bacteria , Gram-positive bacteria and animal tissues .The actual results of the two sample preparation methods were compared by 2-DE.Results The 2-DE maps of samples extracted by the two methods were obtained.Conclusion The 2-DE maps of glass-beads grounding samples are better than those of ultrasonic disruption thanks to their lower backgrounds , which are beneficial for further image analyses .
