Effects of PPARγon malignant arrhythmia in myocardial ischemia/reperfusion model rats
10.11958/j.issn.0253-9896.2015.05.011
- VernacularTitle:PPARγ对大鼠心肌缺血/再灌注后恶性心律失常的影响
- Author:
Xueshu HE
;
Xinling MO
;
Hua CHEN
;
Ting XIE
- Publication Type:Journal Article
- Keywords:
PPAR gamma;
myocardium;
reperfusion injury;
arrhythmia;
rosiglitazone
- From:
Tianjin Medical Journal
2015;43(5):488-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of peroxisome proliferator activated receptor gamma (PPARγ) on malignant arrhythmia in myocardial of ischemia/reperfusion(I/R) rats. Methods Twenty-four SD rats were randomly divided into four groups:Sham group, I/R group, rosiglitazone (ROS) group and PPARγ inhibitor GW9662 (GW) group. The myocardial I/R injury was induced by ligation of the left anterior descending coronary artery, with ischemia for 30 min and reperfusion for 2 h. The whole process limb Ⅱ lead electrocardiogram was applied to observe the frequency of malignant arrhythmia and record the corrected changes of QT(QTc) interval. RT-PCR was used to detect the expression of PPARγ mRNA. Results There were 5 cases of malignant arrhythmia in ROS group, 2 in I/R and 1 in GW group, 0 was found in Sham group. There was a prolongation of the QTc interval in ROS group than the other groups after ischemic stage (P＜0.05). Compared with I/R group and ROS group, the QTc intervals were shorten in ischemia 30 min and reperfusion process in GW group (P＜0.05). Compared with sham group, the expression of PPARγ mRNA was significantly increased in other three groups (P＜0.05). The expression level of PPARγ mRNA was the highest in ROS group. The expression level of PPARγ mRNA was reduced in GW group compared with that of I/R group and ROS group (P＜0.05). Conclusion Over expression of PPARγ may lead to the occurrence of malignant arrhythmia in myocardial ischemia/reperfusion rats.
