Relationship between Polymorphisms of Mitochondrial DNA Displacement-loop and Renal Cell Carcinoma
10.3969/j.issn.0253-9896.2014.03.003
- VernacularTitle:线粒体DNA D-loop基因多态性与肾透明细胞癌关系的研究
- Author:
Shenglei ZHANG
;
Jinsheng XU
;
Yaling BAI
;
Junxia ZHANG
;
Liwen CUI
;
Huiran ZHANG
- Publication Type:Journal Article
- Keywords:
adenocarcinoma,clear cell;
cancer,renal cell;
polymorphism,genetic;
sequence analysis;
DNA,mito-chondria
- From:
Tianjin Medical Journal
2014;(3):200-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between polymorphisms in mitochondrial displacement-loop (mtDNA D-loop) and renal cell carcinoma. Methods Fifty-nine patients with clear cell renal cell cancer (renal cancer group) and 68 healthy control (control group) were selected in this study. The mtDNA D-loop region was amplified and se-quenced using polymerase chain reaction (PCR). Data were compared and analysed with the Revised Cambridge Reference Sequence (rCRS) in library of mitochondria. The difference in frequency analyses of mtDNA D-loop region was compared be-tween two groups. Results A total of 143 single nucleotide polymorphisms (SNP) of mitochondria D-Loop region were de-tected in renal cancer group and control group. Compared with control group, there were significantly higher frequencies of 262T and 16293G alleles in mitochondria D-loop region, and significantly lower frequencies of 16298C and 16319A alleles, in renal cancer group (P<0.05). Conclusion The analysis of genetic polymorphisms in the D-loop can be used as predic-tors of renal cell carcinoma and contribute to the early detection in patients of renal cell carcinoma.
