Comparison of Immune Responses Elicited by Recombinant Fusion Hapten and Coupled Hapten

VernacularTitle:2种方法制备小肽免疫原的免疫特性比较
Author: Ying LIU ; Bei SUN ; Yan MA ; Xueqin ZHAO ; Dongchun LIANG ; Gang GUO ; Jingyu ZHANG
Publication Type:Journal Article
Keywords: recombinant proteins; histocompatibility antigens; haptens; immunity; carrier proteins
From: Tianjin Medical Journal 2009;37(10):862-864
CountryChina
Language:Chinese
Abstract: Objective:To identify the immune characteristics elicited by immunogen prepared in two different ways against a 36AA-peptide. Methods：Rabbits were immunized by a 36-AA-hapten coupled with carrier proteins or recombinant fusion protein separately. The kinetics and specificities of antibody responses were compared. Results：After about 3 months of primary immunity, the antisera elicited by two kinds of immunogen reached the peak titer. The highest titer elicited by the immunogen prepared with coupled-hapten method reached 1∶12 000, while the highest titer elicited by the immunogen prepared with gene engineering method was 1∶6 000. But the immune response produced by the recombinant fusion protein was more long-lasting. Conclusion：Both kinds of immunogen can successfully elicit 36-AA specific antibody response in rabbits, which have different immune characteristics. To couple the hapten with vector proteins is a quicker and more effective way to prepare the immunogen.