Expression of hypoxia inducible factor-1 alpha in human lumbar nucleus pulposus of different herniated types
10.3969/j.issn.2095-4344.2015.29.021
- VernacularTitle:不同突出类型腰椎髓核中缺氧诱导因子1α的表达
- Author:
Xingzhen LIU
;
Zhiyi FU
;
Kangping SHEN
;
Wenjie JIN
;
Yujie WU
- Publication Type:Journal Article
- Keywords:
Intervertebral Disk Displacement;
Spinal Cord;
Hypoxia-Inducible Factor 1
- From:
Chinese Journal of Tissue Engineering Research
2015;(29):4700-4704
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Under hypoxic environment, hypoxia inducible factor-1 plays an important role in regulation of hypoxia-induced gene expression in the intervertebral disc. Hypoxia-inducible factor-1 consists of α and βsubunits, and which hypoxia inducible factor-1α determines the stability and activity of hypoxia-inducible factor-1. OBJECTIVE: To observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus of different herniated types and to judge their relationships. METHODS:A total of 60 nucleus pulposus samples were harvested from the lumbar vertebra, including 41 from L4-5 and 19 from L5-S1, and then divided into protruded group and sequestered group, with 30 cases in each group. Meanwhile, another 10 samples of lumbar nucleus pulposus served as controls. Hematoxylin-eosin staining and streptavidin-biotin peroxidase complex immunohistochemical technique were used to observe the expression of hypoxia inducible factor-1α in the human lumbar nucleus pulposus in different groups. RESULTS AND CONCLUSION:The expression level of hypoxia inducible factor-1α was (58.2±7.5)% in the sequestered group, (27.3±2.3)% in the protruded group, and (10.5±4.7)% in the control group, which was significantly higher in the sequestered group than the other two groups (P < 0.01). These findings indicate that the expression of hypoxia inducible factor-1α in the lumbarnucleus pulposus is associated with the herniated types, which is the highest in the prolapse sequestered type.
