Determination of S-isomer in Epinephrine Hydrochloride Injection by HPLC with Chiral Mobile Phase Additives
10.3870/yydb.2015.06.026
- VernacularTitle:高效液相色谱手性流动相法测定盐酸肾上腺素注射液中 S-对映体含量
- Author:
Li JIN
;
Jianli ZHOU
;
Xiaodan ZHANG
;
Zhuoliang GU
;
Ying BU
- Publication Type:Journal Article
- Keywords:
Epinephrine injection,hydrochloride;
Chromatography,high performance liquid;
Chiral mobile phase;
Additives;
S-isomer
- From:
Herald of Medicine
2015;(6):799-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the chiral separation and determination of S-isomer in epinephrine hydrochloride injection by HPLC with chiral mobile phase additives. Methods Column of Purospher? STAR RP-18 (4. 6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-10 mmol·L-1 sodium dihydrogen phosphate buffer containing 10 mmol·L-1 sulfobutylether-b-cyclodextrin (pH adjusted to 3. 0 with phosphoric acid) (98. 5:1. 5), detection wavelength was 280 nm, the flow rate was 0. 8 mL·min-1 , and the column temperature was 30 ℃ . Results Good linear relationship was established between the peak area and the concentration of S-isomer over the range of 5. 02-1501. 50 μg·mL-1 (R2 =0. 999 7). The detection limit was 0. 05 μg·mL-1 . Conclusion The proposed method shows high repeatability and durability. It can be employed for the quality control of S-isomer in epinephrine hydrochloride injection.
