A Low Noise Amplifier System for Nanopore-based Single Molecule Analysis
10.11895/j.issn.0253-3820.150159
- VernacularTitle:纳米通道单分子检测低噪音电流放大器系统的研究
- Author:
Bingyong YAN
;
Zhen GU
;
Rui GAO
;
Chan CAO
;
Yilun YING
;
Wei MA
;
Yitao LONG
- Publication Type:Journal Article
- Keywords:
Nanopore;
Single molecule detection;
Current amplifier;
Denoising
- From:
Chinese Journal of Analytical Chemistry
2015;(7):971-976
- CountryChina
- Language:Chinese
-
Abstract:
A novel amplifier system was proposed for low-noise recording of pico-ampere current in nanopore experiment (<100 pA). As an example, the amplifier system was applied in α-hemolysin based nanopore detection of DNA-PEG-DNA conjugate to record the signals of translocation and bumping events in buffer solution (1 mol/L KCl, 10 mmol/L Tris--HCl, 1 mmol/L EDTA and pH=8. 0). The amplified current signal was filtered by a 3 kHz Bessel filter and sampled by a 100 kHz analog-digital convertor. As a result, the presented amplifier system could lower the noise in recording the current. The current blockages (<10 pA) of single molecules with low amplitude were recovered due to the high signal-to-noise ratio.
