A murine model of LPS/D-gal-induced acute hepatitis and alterations in mTOR signaling
10.3969/j.issn.1005-4847.2015.03.016
- VernacularTitle:LPS／D－gal诱导小鼠急性肝炎模型及 mTOR 信号的变化
- Author:
Xiaofei LI
;
Yufen TAO
;
Jiansheng LIU
;
Chao LI
;
Xinxin LIANG
;
Yousong YE
;
Donghong TANG
;
Hongqi LIU
- Publication Type:Journal Article
- Keywords:
LPS/D-gal;
Acute hepatitis;
Apoptosis;
Murine model
- From:
Acta Laboratorium Animalis Scientia Sinica
2015;(3):306-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of mTOR signaling in LPS/D-gal-induced acute hepatitis in mice . Methods Twenty-six healthy adult female ICR mice were divided into two groups:the control group and experimental group, 13 mice in each group .LPS/D-gal was used to induce acute hepatitis in the mice .The survival of mice was moni-tored within 24 hours after LPS/D-gal challenge .At 6 hours after challenge , samples of serum and liver tissue were collect-ed for further analysis.Results Injection of LPS/D-gal resulted in acute death of the mice within 24 hours.At 6 hours post LPS/D-gal injection , the blood levels of ALT and AST were significantly increased .The mRNA expression of inflammatory cytokines Tnfa and Il6 was up-regulated in LPS/D-gal-induced hapatitis , in which DNA fragmentation and activation of caspase-3 were subsequently observed .Immunoblot analysis showed that both mTOR pathway and NF-κB pathway were ac-tivated.Unexpectedly , inhibition of mTOR signaling could neither decrease the apoptosis in the liver nor increase the sur -vival of mice .Conclusions The results of the present study indicate that mTOR signaling may play pleiotropic roles in the pathogenesis of LPS/D-gal-induced hepatitis .
