Effect of aging on transcription and protein expressions of procollagen α polypeptide gene of vascular smooth muscle cells in rat
10.3760/cma.j.issn.0254-9026.2015.04.026
- VernacularTitle:增龄对大鼠血管平滑肌细胞前胶原α多肽基因转录和蛋白表达的影响
- Author:
Xiaoan CHEN
;
Tao TIAN
;
Ying WANG
;
Mei LI
;
Yuanyuan RONG
;
Dalin SONG
- Publication Type:Journal Article
- Keywords:
Muscle,smooth,vascular;
Procollagen;
Transcription,genetic
- From:
Chinese Journal of Geriatrics
2015;34(4):438-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of aging on procollagen α polypeptide gene transcription and protein expression in rat vascular smooth muscle cells.Methods Vascular smooth muscle cells from thoracoabdominal aorta in neonate and 9 months old healthy Wistar rats were cultured in vitro.Results Transcription polymerase chain reaction(RT PCR) and Western blotting were used to detect type Ⅰ and Ⅲ pro-collagen α polypeptide mRNA and protein.The RT-PCR displayed that type Ⅰ procollagen α polypeptide mRNA expression had no significant difference between young group and adult group [(76.62±1.05) vs.(78.37±2.42),P＞0.05].Type Ⅲ procollagen α polypeptide mRNA expression was (105.40 ± 2.66) in young group and (123.10 ± 3.81) in adult group(P＞0.05).Type Ⅰ procollagen α polypeptide mRNA expression was (3.13 ±0.54) in young group and (4.63 ± 1.03) in adult group (P=0.05).Type Ⅲ procollagen α polypeptide mRNA expression had no significant difference between the adult and young groups[(6.86 ±0.41) vs.(7.68±0.63),P＞0.05].Type Ⅰ and type Ⅲ procollagen α polypeptide protein expressions were increased significantly in adult group as compared with the young group [(0.10 ± 0.03) vs.(0.06±0.03),(0.58±0.06) vs.(0.40±0.02),both P＜0.05].Conclusions Aging increases the procollagen α polypeptide level in vascular smooth muscle cell,which may involve in the development of vascular remodeling and atherosclerosis.
