The expressions and significances of miR-155 in 52 bone marrow samples of preliminary pediatric acute myeloid leukemia
10.3760/cma.j.issn.2095-428X.2015.09.014
- VernacularTitle:初诊儿童急性髓系白血病52例骨髓miR-155的表达及意义
- Author:
Lihua XU
;
Shaoyan HU
;
Jiannong CEN
;
Hailong HE
;
Hongjie SHEN
;
Dan HONG
- Publication Type:Journal Article
- Keywords:
miR-155;
Marker;
Myeloid leukemia,acute;
Child
- From:
Chinese Journal of Applied Clinical Pediatrics
2015;30(9):694-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differential expression of miR-155 in newly diagnosed pediatric acute myeloid leukemia(AML) and its clinical significances.Methods Fifty-two AML children and 30 non-malignant disease matched children were recruited as the controls.The preliminary AML children were divided into favorable group,moderate group and poor group according to the National Comprehensive Cancer Network(NCCN) 2013.Real-time quantitative polymerase chain reaction was applied to validate the expressions of miR-155 in bone marrow samples (the data presented by 2-△△Ct).Results By comparing expressions of miR-155 between AML patients and controls,the miR-155 expressions were significantly higher in the AML children than those in the controls (Z =-5.391,P ＜ 0.001).There were significant differences among different prognostic groups,with a significantly lower level in the favorable group compared with others (x2 =12.586,P =0.002).It was also found that differential expressions existed not only in kinds of mutation cohort,with the highest level in FLT3-ITD and the lowest one in FLT3-TKD mutation group (x2 =11.216,P =0.024),but also among fusion gene subgroups (x2 =12.254,P =0.016),with the highest level in AML-ETO group and the lowest level in PML-RARa group:meanwhile,the expressions of miR-155 were statistic different according to French-America-British (FAB) subtypes (x2 =17.814,P =0.013),which was lower in M3 patients than non-M3 patients (Z =-3.291,P =0.001).Conclusions It indicates that the expressions of miR-155 may increase sharply in preliminary AML children,and the lower expression of miR-155 is closely related to favorable prognosis.
