In vivo biocompatibility of whole-kidney acellular matrix scaffolds prepared by perfusion method
10.3969/j.issn.2095-4344.2015.16.013
- VernacularTitle:灌注法制备全肾脏脱细胞基质支架的体内生物相容性
- Author:
Jie CHEN
;
Jingqiu YANG
;
Chunxiao LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(16):2529-2533
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The whole-kidney acelular matrix scaffold in rats prepared by perfusion method is proved to have goodin vitro biocompatibility, butin vivo biocompatibility is stil unclear. OBJECTIVE:To produce a whole-kidney acelular matrix scaffold in rats by perfusion method and to evaluate the in vivo biocompatibility of the scaffold. METHODS:The whole-kidney acelular matrix scaffold in Wistar rats was prepared by perfusion method and evaluated with the folowing tests. (1) Acute toxicity test: mice were subject to intraperitoneal injection of whole-kidney acelular matrix scaffold extract liquid, normal saline and phenol. (2) Hemolytic test: Anticoagulant blood samples from New Zealand rabbits were mixed with whole-kidney acelular matrix scaffold extract liquid, normal saline and distiled water, respectively. (3) Pyrogen test: Whole-kidney acelular matrix scaffold extract liquid was injected into the ear vein of New Zealand rabbits. (4) Intracutaneous stimulation test: Whole-kidney acelular matrix scaffold extract liquid was injected subcutaneously into New Zealand rabbits for observing whether there was a skin stimulus response. (5) Subcutaneous implantation test: The whole-kidney acelular matrix scaffold was implanted subcutaneously into the back of New Zealand rabbits. RESULTS AND CONCLUSION:There was no cel residual in the whole-kidney acelular matrix scaffold preparedby perfusion method, and no acute systemic toxicity, hemolytic reaction, pyrogen response, and skin stimulus respons, indicating the scaffold has a good histocompatibility in the rabbits. These findings suggest that the whole-kidney acelular matrix scaffolds in Wistar rats prepared by perfusion method have goodin vivo biocompatibility.
