Effect of SKF96365 and NiCl2 on cyclopiazonic acid induced intracellular calcium cation concentration increase in rat distal pulmonary arterial smooth muscle cells
10.3969/j.issn.1671-8348.2015.11.002
- VernacularTitle:SKF96365和氯化镍对环匹阿尼酸诱导的大鼠PASMC［Ca2＋］i升高的影响
- Author:
Gongyong PENG
;
Jinxing HU
;
Yimin ZOU
;
Fang PENG
;
Yumin ZHOU
;
Guoping HU
;
Zhuxiang ZHAO
- Publication Type:Journal Article
- Keywords:
cyclopiazonic acid;
intracellular calcium cation concentration;
pulmonary arterial smooth muscle cells;
nifedipine;
store-operated Ca2+ channels;
rat
- From:
Chongqing Medicine
2015;(11):1445-1448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of SKF96365 and NiCl2 on cyclopiazonic acid (CPA) induced intracellular calcium cation concentration ([Ca2+ ]i ) change in rat distal pulmonary arterial smooth muscle cells (PASMC) .Methods The rat distal PASMC were isolated and cultured .The effects of CPA ,SKF96365 and NiCl2 on [Ca2+ ]i in PASMC were tested by fluorescence microscope and InCyte [Ca2+ ]i measurement system .Results PASMC were incubated with Ca2+‐free Krebs solution containing 5μmol/L nifedipine ,10 μmol/L CPA caused a small transient increase in [Ca2+ ]i ;after restoration of extracellular Ca2+ to 2 .5 mmol/L ,10 μmol/L CPA caused marked increases in [Ca2+ ]i in PASMC incubated with Krebs solution containing 5 μmol/L nife‐dipine .Both 50 μmol/L SKF96365 and 500 μmol/L NiCl2 distinctly attenuated the increases in [Ca2+ ]i caused by 10 μmol/L CPA in PASMC .However ,neither 50 μmol/L SKF96365 nor 500 μmol/L NiCl2 affected the increases in [Ca2+ ]i caused by 60 mmol/L KCl in PASMC .Conclusion CPA induced increases in [Ca2+ ]i may related to Ca2+ release from sarcoplasmic reticulum and the in‐flux of Ca2+ through store‐operated Ca2+ channels (SOCC) in rat distal PASMC .Both SKF96365 and NiCl2 could selectively block SOCC and attenuated the influx of Ca2+ through SOCC in PASMC .
