RAGE/NF-κB signal pathway in mediating lysophosphatidylcholine-induced TGF-β1 expression in human retinal endothelial cells
10.3969/j.issn.1671-8348.2015.10.008
- VernacularTitle:RAGE／NF-κB 信号通路调控溶血卵磷脂诱导的人视网膜内皮细胞TGF-β1表达
- Author:
Richeng YU
;
Jianhua LUO
;
Yuanshuo FAN
;
Bo LIU
- Publication Type:Journal Article
- Keywords:
lysophosphatidylcholines;
receptor for advanced glycation end products;
human retinal endothelial cells;
transfor-ming growth factor beta1
- From:
Chongqing Medicine
2015;(10):1319-1321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of receptor for advanced glycation end products (RAGE)/NF-κB signaling pathway in mediating lysophosphatidylcholine (LPC)-induced TGF-β1 expression in human retinal endothelial progenitor cells (HEPCs).Methods Human retinal endothelial cells (HERCs)were transfected with siRNA for RAGE siRNA or added NF-κB in-hibitor pyrrolidine dithiocarbamate (PDTC)in the presence or absence of LPC,the expressions of TGF-β1 and RAGE genes were analyzed by qPCR and Western blot.Results LPC could increase the expression of RAGE and TGF-β1 gene in HERCs.The RAGE gene after silence could significantly decrease the expression of LPC-induced RAGE and TGF-β1 .Adding NF-κB inhibitor PDTC significantly reduced LPC-induced RAGE and TGF-β1 expression in HERCs.Conclusion RAGE/NF-κB signaling pathway plays an important role in mediating LPC induced TGF-β1 gene expression in HERCs.
