The study of phosphoinositide-3-kinase inhibitor LY294002 in the differentiation of human embryonic stem cells into more mature insulin-producing cells
10.3969/j.issn.1006-5725.2015.08.005
- VernacularTitle:磷脂酰肌醇3激酶抑制剂LY294002诱导人胚胎干细胞定向分化为胰岛素分泌细胞的研究
- Author:
Yanan WANG
;
Ping LU
;
Xiaohui HUANG
;
Chenguang TIAN
;
Lijun SUN
;
Qian DOU
;
Li TAN
;
Wuliang WANG
;
Genhong MAO
- Publication Type:Journal Article
- Keywords:
Human embryonic stem cells;
Cell differentiation;
Insulin;
Phosphoinositide-3-kinase inhibitor
- From:
The Journal of Practical Medicine
2015;(8):1222-1225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of phosphoinositide-3-kinase inhibitor LY294002 on the differentiation of human embryonic stem cells (HESC) into more mature insulin-producing cells. Methods HESCs were induced to differentiate into insulin-producing cells through five stages. Nicotinamide and B27 (group B27), nicotinamide and LY294002 (group LY) were used to induce the nesting positive cells into mature insulin-producing cells. The morphological change of each stage was observed under microscope , and expressions of insulin, c-peptide, somatostatin and glucagon were identified by immunofluorescence staining. Results After 14 days in stage 5 , there was no significant difference in rate of insulin positive cells between group LY and group B27 (P﹥0.05), but rates of somatostatin and glucagon positive cells in group LY were lower than those in group B27(P﹤0.05). Furthermore, the co-stained rate of somatostatin and insulin in group LY was also lower than that in group B27 (P﹤0.05). Conclusion HESCs can be induced to differentiate into more mature insulin-producing cells by phosphoinositide-3-kinase inhibitor LY294002 in serum-free culture medium.
