Analysis of TCR gene rearrangements for diagnosis of T-cell lymphomas
10.13315/j.cnki.cjcep.2015.04.010
- VernacularTitle:T细胞性淋巴瘤诊断中TCR基因重排检测的应用
- Author:
Xinyan PAN
;
Changshao YANG
;
Guiyun LI
;
Julun YANG
;
Li WANG
- Publication Type:Journal Article
- Keywords:
T-cell lymphomas;
gene rearrangement;
BIOMED-2;
TCR gene
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(4):400-403
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To discuss the TCR gene rearrangements in the diagnosis of T-cell lymphomas. Methods Formalin-fixed and paraffin-embedded samples including 30 cases of T-cell lymphomas and 30 cases of reactive lymphoid hyperplasia were chosen for ex-tracting genomic DNA and PCR amplification using 56 BIOMED-2 primers. PCR products were analyzed by heteroduplex and polyacryl-amide gel electrophoresis. Results In all 30 cases of T-cell lymphomas, 25 cases (83. 3%) showed TCRβ gene monoclonal rear-rangements, 28 cases (93. 3%) of TCRγ gene monoclonal rearrangements, 4 cases (13. 3%) of TCRδ gene monoclonal rearrange-ments. 29 cases (96. 7%) with TCRβ+TCRγ+TCRδ gene monoclonal rearrangements were detected. but no clonal TCR gene rear-rangements were found in 30 cases of reactive lymphoid hyperplasia. Conclusions The detection of TCR gene rearrangements using BIOMED-2 primers is a useful assistant method for the diagnosis of T-cell lymphomas.
