Effect of MCPH1 on irradiation induced DNA damage in esophageal cancer cells

VernacularTitle:MCPH1在电离辐射诱导食管癌细胞DN A 损伤通路中的作用
Author: Baofang WANG ; Baohui WANG ; Xiao XU ; Mingliang CHEN
Publication Type:Journal Article
Keywords: MCPH1; Irradiation; MDC1; DNA double-strand breaks
From: Chinese Journal of Pathophysiology 2015;(5):924-928
CountryChina
Language:Chinese
Abstract: AIM:To discover the effect of MCPH1 on the DNA damage induced by ionizing radiation in esoph-ageal cancer cells.METHODS:ECA109 cancer cells were radiated at dose of 8 Gy.The nuclear foci of relevant factors were detected 1 h after irradiation in the ECA109 cells after silence of MDC1 gene.A cell line was established that was sta-ble low expression of MCPH1.The nuclear foci induced by ionizing radiation after silence of MCPH1 were determined.RE-SULTS:The MCPH1 gene silenced ECA109 cell line was successfully constructed.A strong relationship between MDC1, MCPH1 andγ-H2AX was observed 1 h after 8 Gy irradiation.Silence of MDC1 did not affect the nuclear foci formation ofγ-H2AX and MCPH1.The nuclear foci of MDC1 but notγ-H2AX significantly reduced after silencing of MCPH1.CON-CLUSION:MCPH1 is located in the downstream of H2AX and upstream formation of MDC1, and regulates the nuclear fo-ci formation of MDC1 during DNA damage response.