Effects of modified Sijunzi decoction on the expression of p53 and Bcl-2 in burn-induced esophageal lesion
- VernacularTitle:加味四君子汤对碱烧伤食管组织中p53和Bcl-2表达水平的影响
- Author:
Hongbo SHAO
;
Yueming YAO
;
Qingfu ZHANG
;
Wei WEI
- Publication Type:Journal Article
- Keywords:
modified Sijunzi decoction;
alkali bruning;
esophageal;
p53;
Bcl-2
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish burn-induced esophageal lesion model by adding NaOH with different concentrations in rabbits, and investigate the effect of modified sijunzi decoction on the p53 and Bcl-2.Methods After injection with different concentrations of NaOH, esophagus was dissected and observed anatomically.Rabbits were given a gavage of modified Sijunzi decoction daily for 20 days, and then were injected with NaOH.Esophageal epithelium isolated from each group was observed by hematoxylin-eosin staining.p53 and Bcl-2 protein and mRNA expression was measured with western blot and qRT-PCR, respectively.Results The degree of corrosion of esophageal epithelium was positively correlated with the concentration of NaOH.p53 protein and mRNA levels were increased after NaOH challenge; this increase was inhibited by treatment with modified Sijunzi decoction.Additionally, NaOH decreased Bcl-2 protein and mRNA, which was attenuated by modified Sijunzi decoction.Conclusion Modified sijunzi decoction can relieve the esophageal alkali burning in a dose-dependent manner, suggesting that modified Sijunzi decoction may be a useful strategy to treat chemical injuries in esophageal tissue.
