Inhibitory effect of N-acetyl-seryl-aspartyl-lysyl-proline on epithelial-mes-enchymal transition by heat-shock protein 27/zinc finger proteins
10.3969/j.issn.1000-4718.2015.01.001
- VernacularTitle:Ac-SDKP 经 HSP27调节锌指蛋白而抑制肺上皮细胞-间质转化
- Author:
Haijing DENG
;
Shifeng LI
;
Lijuan ZHANG
;
Xinxin XUE
;
Shipu DU
;
Yue SUN
;
Hong XU
;
Fang YANG
- Publication Type:Journal Article
- Keywords:
N-acetyl-seryl-aspartyl-lysyl-proline;
TypeⅡalveolar epithelial cells;
Myofibroblasts;
Heat shock protein 27;
Zinc finger protein SNAI1;
Zinc finger protein SNAI2
- From:
Chinese Journal of Pathophysiology
2015;(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect whether N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) inhibits epithelial-mes-enchymal transition in A549 cells induced by TGF-β1 through suppressing the expression of heat shock protein 27 (HSP27) and zinc finger proteins Snail (including SNAI1and SNAI2) which ultimately inhibited the deposition of type I and type III collagens.METHODS:The colocalizations of HSP27 and SNAI1/SNAI2 respectively on A549 alveolar epi-thelial cells induced by TGF-β1 were measured by confocal microscopy .The expression of HSP27, SNAI1 and SNAI2 at mRNA level was detected by real-time PCR.Western blotting analysis was used to detect the expression of HSP 27, SNAI1 and SNAI2 on epithelial-mesenchymal transition in A549 cells induced by TGF-β1 and also the deposition of type I and type III collagens in A549 cells transfected with HSP27shRNA prior to TGF-β1 stimulation.RESULTS: Compared with control group, TGF-β1 increased the expression of HSP27, SNAI1, SNAI2, type I and type III collagen, which decreased significantly followed by Ac-SDKP intervention.The expression of SNAI1, type I and type III collagen decreased signifi-cantly after transfected with HSP27shRNA in A549 cells, which had the similar effect on Ac-SDKP intervention.CON-CLUSION:Ac-SDKP inhibits the transition of cultured A 549 cells to myofibroblasts and attenuates collagen synthesis by suppressing the expression of HSP 27 and zinc finger proteins SNAI 1 and SNAI2.
