Effect of Mongolian medicine Senden decoction on IFN-γexpression of CIA model rats
10.3760/cma.j.issn.1008-6706.2015.14.006
- VernacularTitle:蒙药森登汤对胶原诱导性关节炎模型大鼠干扰素-γ表达的影响
- Author:
Mandula NAREN
;
Qiumei DONG
- Publication Type:Journal Article
- Keywords:
Arthritis,Experimental;
Models,Animal;
Interferon-gamma
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(14):2097-2100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Mongolian medicine Senden decoction on IFN-γexpression of CIA model rats.Methods 10 rats were randomly selected as the normal group.The other 50 SD rats were established CIA model,and were randomly divided into 5 groups:the model group,Mongolian medicine morito decoction of high, medium and low dose group,tripterygium glycosides group.At different time points,the arthritis index,immunohisto-chemical expression of IFN-γwere detected in the synovial cytokine,the protein expression of IFN-γin jaw joint was detected by Western blot.Results Mongolian Senden decoction could improve CIA rat arthritis index,improve the IFN-γpositive expression in synovial joints, reduce the expression of IFN -γprotein in the claw gamma, tripterygium wilfordii group and Mongolian senden decoction high dose group improved the most obviously,and there was significant difference compared with model group(FAI 40d=27.276,FIFN-γPositive =32.654,FIFN-γprotein =674.124, all P=0.000).Conclusion Mongolian medicine Senden decoction can improve symptoms and IFN-γjoint inflam-matory cytokine expression in rat CIA model,the effect of joint synovial membrane,and the positive control medicine tripterygium glycosides, but there is a dose -response relationship, and low doses of Mongolian medicine senden decoction high dose effect is better than that of medium and low dose.
