Total saponins of panax ginseng enhances the effect of osteoblast differentiation from mesenchymal stem cell on promoting hematopoiesis
10.3969/j.issn.1001-1978.2015.01.011
- VernacularTitle:人参总皂苷增强成骨分化的间充质干细胞促造血作用研究
- Author:
Liming YIN
;
Yanna ZHAO
;
Wenxi DU
;
Xiao WANG
;
Like WO
;
Ruilan GAO
- Publication Type:Journal Article
- Keywords:
total saponins of panax ginseng;
mesen-chymal stem cell;
osteoblast;
hematopoietic microenvi-ronment;
differentiation;
hematopoiesis
- From:
Chinese Pharmacological Bulletin
2015;(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe mesenchymal stem cell differentiation into osteoblast induced by TSPG, and explore how TSPG enhances the promotion of hemato-poiesis of osteoblast differentiation from mesenchymal stem cell. Methods MSCs were cultured by TSPG combined with osteoinductive medium. The cellular vi-ability of proliferation was detected with MTT assay. The content of alkaline phosphatase in the cultural su-pernatant was tested with pNPP assay. The ability of MSCs to form calcium nodes was also observed after a-lizarin red stain. The protein expression of RUNX2 was analyzed with Western blot. The content of cytokines associated with hematopoiesis was tested with Elisa as-say. The ability of promoting hematopoiesis was detec-ted with hematopoietic colony forming assay. Results Both MTT and pNPP assay showed that optical den-sity ( OD) values were increased in response to TSPG treatment in a dose-dependent manner. The mineraliza-tion formation ability was enhanced with TSPG-treat-ment. Meanwhile, the expression of RUNX2 protein was up-regulated in TSPG-treated cell. Moreover, the content of cytokines associated with hematopoiesis and the number of hematopoietic progenitor colony were in-creased by TSPG-treatment compared with the control group. Conclusion TSPG could induce MSCs differ-entiation in to osteoblast and enhance the effect of oste-oblast differentiation from MSCs on promoting hemato-poiesis.
