Phosphoprotein EBP50 suppresses proliferation of breast cancer by inhibiting activity of ERK1/2 in MCF-7 cell line

VernacularTitle:磷酸化蛋白EBP50通过降低ERK1/2活性抑制乳腺癌细胞MCF-7增殖能力
Author: Hong LIU ; Yan MA ; Rongguang SHAO
Publication Type:Journal Article
Keywords: EBP50; ERK1/2; MCF-7; breast canc-er; proliferation; pGPU6/Neo
From: Chinese Pharmacological Bulletin 2015;(1):55-59
CountryChina
Language:Chinese
Abstract: Aim To investigate the relationship be-tween phosphoprotein EBP50 and the proliferation of breast cancer in MCF-7 cells. Methods The quali-fied recombinant plasmid sh-EBP50-pGPU6/Neo was transfected into MCF-7 cells with EBP50 knocking down. The expression of EBP50, c-myc, p-ERK1/2, and ERK1/2 was detected by Western blot. The prolif-eration ability of cells was detected by sulforhodamine B assay. Results The EBP50 knocking down plasmid was constructed successfully. MCF-7 cells with EBP50 knocking down had been established successfully. Knocking down of EBP50 increased the proliferation of MCF-7 significantly, and partially augmented the ex-pression of c-myc and phosphorylation of ERK1/2 . However, knocking down of EBP50 did not impact the expression of ERK1/2 . Conclusion EBP50 suppres-ses the proliferation of breast cancer cell through inhib-iting the activity of ERK1/2 in MCF-7 cell line.