Immunoregulation effect of TIGIT-Fc on macrophages
10.3969/j.issn.1000-484X.2015.02.015
- VernacularTitle:TIGIT-Fc融合蛋白的表达以及对巨噬细胞功能的影响
- Author:
Xia ZHAO
;
Xi CHEN
;
Zhelong LIU
;
Hui CHENG
;
Wentao HE
- Publication Type:Journal Article
- Keywords:
TIGIT;
Macrophage;
PVR;
IL-10
- From:
Chinese Journal of Immunology
2015;(2):210-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare the production of TIGIT-Fc fusion protein using H22 cells stably integrated the gene by lentivirus vector , and to explore the immunoregulatory effect on macrophages by TIGIT-Fc.Methods: TIGIT-Fc fusion gene were constructed by molecular cloning.The fusion gene was then subcloned to plasmids contained the secretion signaling peptide .The secrected TIGIT-Fc fusion gene was inserted into the lentivirus backbone vector.The purified lentivirus vector was the used to infect the murine H22 cell line.TIGIT-Fc protein was purified by protein A column from the ascites of H 22-injected C57BL/6 mice.Macrophages stimulated by lipopolysaccharide ( LPS ) was challenged to TIGIT-Fc treatment or control.Cytokine levels was then detected by ELISA.Results: TIGIT-Fc protein was purified from the ascites of H 22-injected mice.PVR was upregulated in LPS-treated macrophages.IL-10 level was upregulated in TIGIT-Fc treated macrophages.Conclusion: TIGIT-Fc promotes the mature macrophages to secrete anti-inflammatory cytokine IL-10.
