Establishing mouse models of allergic rhinitis by knocking outH2-eb1 gene
10.3969/j.issn.2095-4344.2015.27.028
- VernacularTitle:敲除H2-eb1基因建立的变应性鼻炎模型小鼠
- Author:
Linge LI
;
Juan FENG
;
Bin HU
;
Xi SHOU
;
Chun ZHANG
;
Yu TIAN
;
Chunrong JIANG
;
Yu ZHANG
;
Hua ZHANG
- Publication Type:Journal Article
- Keywords:
Rhinitis,Alergic,Perennial;
Gene Knockout Techniques;
Inbreeding;
Th1/Th2 Balance Funding:the National Natural Science Foundation of China,No. 81160125
- From:
Chinese Journal of Tissue Engineering Research
2015;(27):4417-4422
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:HLA-DRB1 is related to the pathogenesis of alergic rhinitis. Construction ofHLA-DRB1 gene knockout animal models not only elucidates the pathogenesis of alergic rhinitis, but also provides a good way for the elucidation of the pathogenesis of alergic rhinitis-related diseases. OBJECTIVE:To establish the HLA-DRB1gene knockout animal models. METHODS:Homozygous, wild-type and heterozygous mice were obtained by inbreeding of the heterozygous mice. Confirmed by gene and protein identification, 24 female wild-type (H2-eb1+/+) mice and 12 H2-eb1-/-mice aged 8 weeks were selected according to the random number table. 12 H2-eb1+/+ mice and 12 H2-eb1-/- mice were sensitized with ovalbumin to establish the mouse models of alergic rhinitis. Another 12 mice were sensitized with PBS as comparison. RESULTS AND CONCLUSION:Compared with the control mice, serum levels of ovalbumin-specific IgE and interleukin-4 were significantly increased, while serum level ofγ-interferon was significantly decreased in the mouse models of alergic rhinitis. Serum levels of IgE and interleukin-4 were lower, while serumγ- interferon level was higher, inH2-eb1-/-gene knockout mice of alergic rhinitis than those in the H2-eb1+/+ gene knockout wild-type mice. These results suggest thatH2-eb1 gene may play an important role in regulating Th1/Th2 imbalance in the pathogenesis of alergic rhinitis.
