PCR-ribotyping for genotyping Clostridium dif ficile clinical isolates
10.3969/j.issn.1673-4130.2015.06.033
- VernacularTitle:PCR-核糖体分型技术基因分型艰难梭菌
- Author:
Kelin XIAO
;
Ping JIN
;
Liqing HUANG
;
Xia LIANG
;
Tianxiang ZHOU
;
Zhongxing WANG
;
Qinning WANG
;
Fanrong KONG
- Publication Type:Journal Article
- Keywords:
Clostridium difficile;
ribotype;
toxin A and B
- From:
International Journal of Laboratory Medicine
2015;(6):793-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotype and production of toxin A and B of C .difficile clinical isolates collected from Sydney ,Australia .Methods Sixty‐eight C .difficile clinical isolates were collected from Westmead Hospital ,the University of Sydney ,which were genotyped by using PCR‐ribotyping ,and toxin A ,B coding gene tcdA ,tcdB were detected by using PCR meth‐od .Results Thirty‐one PCR‐ribotypes (RTs) were confirmed in the 68 C .difficile clinical isolates ,RT014 (19 .1% ) and RT002 (11 .8% ) were the common genotypes .Sixty‐four of 68 (94 .1% ) isolates contained tcdA and tcdB for toxin A and B .Conclusion The common prevalent PCR‐ribotypes of C .difficile were RT014 and RT002 in Sydney ,most of the C .difficile clinical isolates contained toxin A and B .
