Caudatin sensitizes TRAIL-induced HepG2 cell apoptosis
10.3969/j.issn.1000-4718.2015.02.016
- VernacularTitle:告达庭增强 TRAIL 诱导的 HepG2细胞凋亡
- Author:
Hongrong FEI
;
Guiling WANG
;
Ying ZHAO
;
Honglei ZHOU
- Publication Type:Journal Article
- Keywords:
Caudatin;
Tumor necrosis factor-related apoptosis-inducing ligand;
Carcinoma,hepatocellular;
Apoptosis;
Caspases
- From:
Chinese Journal of Pathophysiology
2015;(2):279-283
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine whether caudatin , a C21 steroidal aglycone , enhances tumor necrosis factor-re-lated apoptosis-inducing ligand ( TRAIL)-associated HepG2 cell apoptosis .METHODS:Cell growth inhibition was deter-mined by MTT assay and cell colony formation assay .The TUNEL apoptosis detection kit was used to analyze cell apopto-sis, and the protein expression was examined by Western blotting .RESULTS:Combination of caudatin with TRAIL signi-ficantly reduced cell proliferation and increased the apoptotic rate of HepG 2 cells compared with the use of each agent alone.This was evidenced by marked increases in caspase-3, caspase-7, caspase-9 and PARP cleavages in the cells treated with caudatin and TRAIL-compared with control group .Combination of caudatin with TRAIL also led to the strong suppres-sion of survivin .CONCLUSION:Caudatin synergizes HepG 2 cells to TRAIL-induced apoptosis by promoting the cleava-ges of caspase-3, caspase-7, caspase-9 and PARP and inhibiting the expression of survivin .
