Tumor-associated Neovascularization in C6 Glioma Tumor Margin Measured with Dynamic Contrast-enhanced MRI
10.3969/j.issn.1005-5185.2015.02.001
- VernacularTitle:动态对比增强MRI评价大鼠C6胶质瘤肿瘤边缘新生血管的实验研究
- Author:
Xuesong DU
;
Heng LIU
;
Xiao CHEN
;
Jingqin FANG
;
Shunan WANG
;
Weiguo ZHANG
- Publication Type:Journal Article
- Keywords:
Glioma;
Magnetic resonance imaging;
Image enhancement;
Neovascularization,pathologic;
Disease models,animal;
Rats,Sprague-Dawley
- From:
Chinese Journal of Medical Imaging
2015;(2):81-86
- CountryChina
- Language:Chinese
-
Abstract:
PurposeTo investigate the formation of neovascularization using dynamic contrast-enhanced MRI (DCE-MRI) in C6 glioma margin in rats, and to explore its utility.Materials and MethodsC6 glioma model was created in 24 SD rats. Routine and DCE-MRI was performed 4 days, 8 days, 12 days, 16 days, 20 days and 24 days after transplantation. Pathology and immunohistochemistry exams were also obtained to evaluate marginal tumor cells and neovascularization.ResultsNeovascularization was observed in C6 glioma margin within (257.658±53.663)μm range. Vessel co-option index difference was statistically significant (P<0.01). Tumor cells surrounding the co-opted vessels were strongly positive on proliferating cell nuclear antigen and matrix metallopeptidase 2 staining. Vessel co-option regression and transformation were observed on day 16 on Tenascin-C andα smooth muscle actin staining. For DCE-MRI analysis, Ktrans and Kep were higher on day 12 and 16 (P<0.05). There was significant negative correlation between co-option index and Ktrans (r=-0.9243 and-0.8347,P<0.05). Vp showed no statistically significant difference at all time points (P>0.05).Conclusion The neovascularization in tumor margin demonstrates vessel co-option. DCE-MRI parameters Ktrans and Kep are negatively correlated with co-option index, indicating that Ktrans and Kep could be a well-defined imaging-based biomarker for glioma neovascularization.
