Etiologic diagnosis and analysis of 8 cases of human campylobacteriosis in Guizhou Province, China
10.3969/cjz.j.issn.1002-2694.2014.10.020
- VernacularTitle:贵州省8例空肠弯曲菌病例的病原学诊断与分析
- Author:
Xiaoyu WEI
;
Shijun LI
;
Lu YOU
;
Kecheng TIAN
;
Guangpeng TANG
;
Dingming WANG
- Publication Type:Journal Article
- Keywords:
Campylobacter jejuni;
Guizhou;
PCR;
PFGE
- From:
Chinese Journal of Zoonoses
2014;(10):1079-1081
- CountryChina
- Language:Chinese
-
Abstract:
Eight patients with suspected cases of C .jejuni were etiologically diagnosed and analyzed in this study to pro-vide scientific basis for the confirmation of the cases of human campylobacteriosis in Guizhou Province ,China .Blood or feces of 8 suspected patients were employed to isolate bacteria strains .Conventional and multi-PCR techniques were applied to identify suspicious bacteria strains .The C .jejuni strains were analyzed by using Pulsed Field Gel Electrophoresis (PFGE) .Suspicious strains of C .jejuni were isolated from all the 8 suspected patients of campylobacteriosis and anticipated genes fragment were detected with multi-PCR .With the digestion of restriction enzyme SmaI ,the 8 C .jejuni strains were divided into 7 PFGE pat-terns with 7-10 DNA bands .Cluster analysis showed that the gross similarity of 8 strains of C . jejuni was more than 50% . The similarity of PFGE patterns between strain GZ201004 and GZ201005 from diarrhea patients was as high as 100% ,while the similarity of strain GZ201201 and GZ201007 was 66 .7% .Moreover ,C . jejuni were detected from all the suspected pa-tients of campylobacteriosis .PFGE results indicated that strains GZ201004 and GZ201005 were from the same source ,while all the 8 isolates showed PFGE polymorphism .
