Promoting effects of Guavenoic acid (GA)on proliferation,insulin synthesis and secretion in INS-1 cells and their mechanism
10.3969/j.issn.1001-1978.2014.12.013
- VernacularTitle:番石榴酸对 INS-1胰岛β细胞增殖、胰岛素合成与分泌的促进作用及其机制分析
- Author:
Kaihe YE
;
Jingru WANG
;
Jinjin MA
;
Xiaokang WANG
;
Xiaoqi ZHANG
;
Wencai YE
;
Chunling YE
- Publication Type:Journal Article
- Keywords:
Guavenoic acid;
INS-1 βcells;
prolifera-tion;
insulin synthesis;
insulin secretion;
gene expres-sion
- From:
Chinese Pharmacological Bulletin
2014;(12):1681-1687
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Guaveno-ic acid (GA)on proliferation,insulin synthesis and secretion in INS-1 cells and their possible mechanism. Methods INS-1 βcells were cultured in vitro.Control group,medium group,model group,drug groups and positive group were set.INS-1 cells were treated with GA (0.3,1 ,3,1 0,30 nmol·L -1 )for 48 h.The cell proliferation was tested by MTT assay.Acid-alco-hol was used to extract the insulin in the cells and the amount of insulin synthesis of INS-1 cells was tested by RIA.5.6,1 6.7 mmol·L -1 glucose was used to chal-lenge INS-1 cells for 1 h to the insulin secretion model (BIS and GSIS)was tested,and the insulin secretion of INS-1 cells was tested via RIA.The mRNA expres-sion of insulin gene,PDX-1 and MafA was tested by q-PCR.Results Compared with medium group,GA could promote the proliferation of INS-1 cells signifi-cantly (P <0.01 )and promote the synthesis of insulin in INS-1 cells significantly (P <0.01 ).GA(0.3 ~30 nmol· L -1 )could promote the BIS,GSIS of INS-1 cells significantly (P <0.05 or P <0.01 ).GA (3,30 nmol·L -1 )could up-regulate the mRNA expression of insulin gene,PDX-1 ,MafA in INS-1 cells signifi-cantly (P <0.01 ).Conclusions GA could signifi-cantly improve the proliferation of INS-1 cells and pro-mote the insulin synthesis and secretion of INS-1 cells, which may be associated with up-regulation of insulin gene,PDX-1 ,MafA mRNA expression.
