Mechanism of ginsenoside Rh2 inhibiting HepG2 cells migration
10.3969/j.issn.1000-484X.2015.01.013
- VernacularTitle:人参皂苷Rh2抑制肝癌HepG2细胞迁移的实验研究
- Author:
Ziqiang FENG
;
Guowei ZUO
;
Qingqiang SHI
;
Lücui ZHAO
;
Nian LUO
;
Zhimei YOU
;
Jing XIA
;
Danyang LI
;
Jing LI
;
Dilong CHEN
- Publication Type:Journal Article
- Keywords:
HepG2 cells;
Rh2;
Migration;
MAPK;
AP1;
MMP3
- From:
Chinese Journal of Immunology
2015;(1):61-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of ginsenoside Rh2 inhibiting HepG2 cells migration.Methods:HepG2 cells in logarithmic growth phase were cultured in 96-well plates,which were induced by different concentration Rh2,respectively for 24,48,72 hours.The cell inhibition was detected by Cell Counting Kit.Transwell chambers was used to checked HepG2 cell migration ability;luciferase was tested by Luciferase Reporter Assay system reagent;The expressions of P-ERK,ERK,P-P38,P-38,P-JUK,JUK,MMP3 proteins were detect by Western blot;the expression of AP1,MMP3 gene were detected by Quantitative PCR;The expression of AP1, MMP3 fluorescence protein were observed by fluorescence microscopy.Results:Administrated with different concentration of Rh2 after 24 ,48 ,72 h,the proliferation of HepG2 cells were inhibited ( P<0.05) ,and in dose-and time-dependent manner.Transwell assay showed Rh2 could significantly inhibited migration of HepG2 cells.The expressions of P-ERK , MMP3 proteins were significantly decreased,the expressions of P-JUK, P-P38 proteins were significantly increased, expression levels of ERK, P-38, JUK were no significant difference.Expression of AP1,MMP3 gene were significantly decreased,the expressions of AP1,MMP3 fluorescence proteins were significantly decreased.Conclusion:Ginsenoside Rh2 can activate MAPK pathway to inhibit the migration of HepG2 cells.
