Effect of recombinant Elafin on A549 apoptosis induced by paraquat

VernacularTitle:转染Elafin对百草枯诱导的肺泡上皮凋亡的影响
Author: Kaixiu QIN ; Jianchun ZONG ; Yan FANG ; Xiaolong WANG
Publication Type:Journal Article
Keywords: Paraquat; A549; Apoptosis; Elafin; Nuclear factor erythroid like-2
From: Chinese Journal of Immunology 2015;(1):45-47
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of recombinant Elafin on A549 apoptosis induced by paraquat and the underlying mechanism.Methods:pEGFP-C1-Elafin was transformed into A549 competent cells by electroporation.Transformed A549 was cultured for additional 24 h before Elafin mRNA was detected by RT-PCR and cocultured with or without various concentration of paraquat( PQ ) for different duration.A549 apoptosis percentage and reactive oxygen species ( ROS ) content were tested by flow cytometry .Nuclear factor erythroid like-2(Nrf2) heme oxygenase-1(HO-1) were examed by Western blot .Results:Elafin expressed successfully in A549 after transformation.PQ caused A549 apoptosis in a concentration dependent manner and leaded to ROS content increasing and Nrf2 and HO-1 decreasing.However,elafin could inhibit ROS production and A549 apoptosis,upregulate Nrf2 and HO-1.Conclusion:Elafin could restrict A549 apoptosis to some extent and the possible mechanism lied in its ability to upregulate Nrf2 ex-pression.