Effects ofSimiaosan on CTGF, BMP-7 Expression in Human Renal Tubular Epithelial Cell Induced by Uric Acid

VernacularTitle:四妙散对尿酸诱导人肾小管上皮细胞结缔组织生长因子及骨形成蛋白-7表达的影响
Author: Jie KANG ; Jicheng CHEN ; Lingyuan ZHANG ; Shanshan DING
Publication Type:Journal Article
Keywords: Simiaosan; uric acid; human renal tubular epithelial cell; fibrosis; epithelial- myofibroblast transdifferentiation
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(1):73-76
CountryChina
Language:Chinese
Abstract: Objective;To explore the mechanism ofSimiaosan drug-containing serum on prevention and treatment of renal fibrosis.Methods Human renal tubular epithelial cells (HK-2) were divided into blank group, model group, control group, and drug-containing serum high dosage group, medium dosage group and low dosage group. The expressions of CTGF and BMP-7 were determined by real-time PCR assay and immunocytochemistry.Results After the HK-2 cell was induced by uric acid (UA), the expression of CTGF significantly increased (P<0.05), the expression of BMP-7 significantly decreased (P<0.05). After intervened bySimiaosan, the expression of CTGF significantly decreased (P<0.05), the expression of BMP-7 significantly increased (P<0.05) and present dosage dependent.Conclusion Reducing the expression of CTGF and up-regulation the expression of BMP-7 and controlling epithelial-myofibroblast transdifferentiation may be the mechanism ofSimiaosan on prevention and treatment of renal fibrosis.