Regulatory fe fect of Hsf1 on PLC/PRF5 hepa toma cells proliferation
10.3969/j.issn.1000-484X.2014.12.004
- VernacularTitle:Hsf1对人肝癌细胞株PLC／PRF5的生长调控作用
- Author:
Qiying JIANG
;
Zhi ZHANG
;
Mingli WANG
;
Yuanfang MA
- Publication Type:Journal Article
- Keywords:
Heat shock transcription factor 1( Hsf1);
Hepatoma cell;
p53;
Rb
- From:
Chinese Journal of Immunology
2014;(12):1601-1605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the regulatory effect of Hsf 1 on PLC/PRF5 hepatoma cells proliferation.Methods: By shRNA gene silencing technology ,constructed PLC/PRF5 hepatoma cell line of Hsf 1 gene silencing.To detect the expression of Hsf 1, p53 and Rb proteins in PLC/PRF5 hepatoma cells by Western blot.The proliferation of PLC/PRF5 cell line was observed by methylthiazolyl tetrazolium assay ( MTT ) , plate clone formation assay ( PCFA ) and cell cycle assay.Results: shRNA-Hsf1 could significantly inhibit the expression of Hsf 1 in PLC/PRF5 cells.It could induce PLC/PRF5 cells stopping at G1 phase of cell cycle , inhibit cell proliferation and colonal formation;silencing Hsf1 caused up-regulation of p53 and Rb proteins expression in PLC/PRF5 cells.Conclusion: Silencing Hsf1 is involved in up-regulation of p53 and Rb proteins expression , which results in inhibiting proliferation of PLC/PRF5 hepatoma cells.
