Resveratrol inhibits chondrosarcoma via mitochondrial and PI3 K/Akt signaling pathways
10.3969/j.issn.1000-4718.2014.08.007
- VernacularTitle:白藜芦醇通过 PI3 K／Akt通路及线粒体途径抑制软骨肉瘤
- Author:
Haidong JIN
;
Bing DAI
;
Jianwu CAI
;
Hui CHEN
;
Shiyang FAN
;
Jun PAN
- Publication Type:Journal Article
- Keywords:
Chondrosarcoma;
Resveratrol;
PI3K/Aktpathway;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2014;(8):1379-1383
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:Toinvestigatetheinhibitoryeffectsofresveratrolonchondrosarcomaandtherelationwith mitochondrial and PI3K/Akt pathways.METHODS:Chondrosarcoma SW1353 cells were treated with resveratrol at con-centrations of 25, 50 and 100 μmol/L for the time intervals of 24 h, 48 h and 72 h.The viability and apoptosis of the SW1353 cells in the presence or absence of resveratrol were analyzed by CCK 8 assay and Hoechst 33258 staining , respec-tively.The protein levels of Bcl-2, Bax, activated caspase-3, Akt and p-Akt were detected by Western blotting .The cell migration ability was determined by wound scratch assay .RESULTS:Exposure of the cells to resveratrol resulted in a de-crease in the cell viability in a dose-and time-dependent manner (P<0.05).visible nuclei with apoptotic characteristics in resveratrol group were observed .The protein levels of activated caspase-3 and Bax were increased , and Bcl-2 and p-Akt were decreased compared with control group .The total Akt were not significantly changed .Resveratrol also significantly re-duced the migration of tumor cells .CONCLUSION:Resveratrol induces apoptosis of chondrosarcoma , which plays a role of part through mitochondrial and PI 3K/Akt signaling pathways .
